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Phd Position
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(New) PhD Position In Quantum Networks
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PhD position
4 maanden geleden
PhD Research project
As part of this new and exciting research program at TU Delft, we are seeking to hire a PhD student to contribute to the development of CA, focusing on the engineering of mammalian cell lines with desirable properties for upscaled cultivated meat production. Such properties include immortalisation, capacity for extreme-density cultivation, resistance to cellular stresses (for example, shear stress induced by agitation), and proliferation in minimal media (with respect to both growth factors, and basic nutrient sources).
The PhD candidate will design and optimise strategies for the engineering of such phenotypes into mammalian cells from agriculturally relevant species, using a variety of genetic modification and gene editing tools, including CRISPR/Cas9. Initial projects might include the development of an immortal and apoptosis-resistant muscle cell line. Attention will be paid to the methods used, to ensure industry-relevance in a complex regulatory landscape. For example, precision nuclease-, base- and prime-editing techniques will be employed where appropriate. The candidate will also combine gene edits/modifications to produce candidate cell lines for subsequent screening in upscaled culture systems, in collaboration with other members of the group and department. This work will contribut e to the development of integral process concepts for CA products based on mammalian cell culture.
Key focus points:
- Gene editing for the engineering of new or improved phenotypes into a variety of mammalian cell lines.
- Characterization of novel cell lines with a variety of techniques including cell-based assays, microscopy and flow cytometry.
- Collaboration with Bioprocess Engineers for the testing of promising cell lines in upscaled culture systems.
You will have the opportunity to further develop your scientific career by 1) becoming an expert in cutting edge genetic modification and gene editing techniques, 2) expanding your skill set with various cell biological techniques, and 3) honing the ability to apply creativity and conceptual thinking to answer pressing research questions. In addition, you will participate in educational activities and gain experience as a supervisor of BSc/MSc research projects.
Research group
This position will be embedded in the Bioprocess Engineering section at the Department of Biotechnology, within the research group of Joshua Flack (Cellular Engineering). Close collaboration is envisaged with other groups within the BPE section, including Marieke Klijn (Data-driven Bioprocess Development) and Marcel Ottens (Bioprocess Engineering). The Bioprocess Engineering section has mammalian cell culture, advanced bioprocess monitoring, fermentation and process design expertise present (USP & DSP). The research is part of the national NGF CA program.
Preferred start date: September 2024
Requirements
The ideal candidate will be an ambitious, proactive, reflective, and results-driven researcher. We are looking for the following:
- MSc degree in a field relevant to the subject, such as Cell Biology, Biochemistry or B iochemical E ngineering .
- Prior laboratory experience with mammalian cell culture is essential.
- Experience with standard molecular biology and cloning techniques is essential.
- Experience with gene editing techniques (such as CRISPR/Cas9) is highly desirable.
- A strong interest in cellular agriculture/cultivated meat is essential, although specific prior experience in this area is not required.
- This is a new line of research at TU Delft, and the candidate should feel excited to establish new methodologies, protocols and readouts within the research group.
- Strong teamwork skills and confidence to take the lead in collaborations.
- Fluen cy in English (written and spoken).
- Strong communication/reporting and presentation skills
[insert requirements from form here, please make sure to make sure that the text below about language requirements is NOT removed]
Doing a PhD at TU Delft requires English proficiency at a certain level to ensure that the candidate is able to communicate and interact well, participate in English-taught Doctoral Education courses, and write scientific articles and a final thesis. For more details please check the .
Conditions of employment
Doctoral candidates will be offered a 4-year period of employment in principle, but in the form of 2 employment contracts. An initial 1,5 year contract with an official go/no go progress assessment within 15 months. Followed by an additional contract for the remaining 2,5 years assuming everything goes well and performance requirements are met.
Salary and benefits are in accordance with the Collective Labour Agreement for Dutch Universities, increasing from € 2770 per month in the first year to € 3539 in the fourth year. As a PhD candidate you will be enrolled in the TU Delft Graduate School. The TU Delft Graduate School provides an inspiring research environment with an excellent team of supervisors, academic staff and a mentor. The Doctoral Education Programme is aimed at developing your transferable, discipline-related and research skills.
T he TU Delft offers a customisable compensation package, discounts on health insurance, and a monthly work costs contribution. Flexible work schedules can be arranged.
For international applicants, TU Delft has the . This service provides information for new international employees to help you prepare the relocation and to settle in the Netherlands. The Coming to Delft Service offers a for partners and they organise events to expand your (social) network.
TU Delft (Delft University of Technology)
Delft University of Technology is built on strong foundations. As creators of the world-famous Dutch waterworks and pioneers in biotech, TU Delft is a top international university combining science, engineering and design. It delivers world class results in education, research and innovation to address challenges in the areas of energy, climate, mobility, health and digital society. For generations, our engineers have proven to be entrepreneurial problem-solvers, both in business and in a social context.
At TU Delft we embrace diversity as one of our core and we actively to be a university where you feel at home and can flourish. We value different perspectives and qualities. We believe this makes our work more innovative, the TU Delft community more vibrant and the world more just. Together, we imagine, invent and create solutions using technology to have a positive impact on a global scale. That is why we invite you to apply. Your application will receive fair consideration.
Challenge. Change. Impact
Faculty Applied Sciences
With more than 1,100 employees, including 150 pioneering principal investigators, as well as a population of about 3,600 passionate students, the Faculty of Applied Sciences is an inspiring scientific ecosystem. Focusing on key enabling technologies, such as quantum- and nanotechnology, photonics, biotechnology, synthetic biology and materials for energy storage and conversion, our faculty aims to provide solutions to important problems of the 21st century. To that end, we educate innovative students in broad Bachelor's and specialist Master's programmes with a strong research component. Our scientists conduct ground-breaking fundamental and applied research in the fields of Life and Health Science & Technology, Nanoscience, Chemical Engineering, Radiation Science & Technology, and Engineering Physics. We are also training the next generation of high school teachers.
Click to go to the website of the Faculty of Applied Sciences .
The position is available in the Department of Biotechnology,
