Transmembrane Protein Mimics for Communication

3 weken geleden

Amsterdam Oost, Nederland AMOLF Voltijd

**Transmembrane protein mimics for communication between synthetic cells**:
**Work Activities**:
More about the project

Functionalizing synthetic cells with transmembrane proteins is still a technical bottleneck. You will circumvent the need to solve this technical challenge time and again by generating a modular toolbox for trans-membrane protein mimics - an approach centered around transmembrane peptides. The central goal is to have a protocol for firstly integrating a transmembrane peptide in lipid vesicles (phase-transfer, microfluidics), and then functionalizing it in a second step with an extracellular component for signal sensing and an intracellular component for signal propagation via bio-orthogonal chemistries. In short: you will work on bio-inspired communication across synthetic membranes without the need for laborious transmembrane protein purification/insertion or transport across the membranes. The modular “LEGO” approach means that it would open a general route for generating bespoke artificial receptors for bottom-up synthetic biology in the future. In order to answer these questions, you will integrate methods from synthetic biology and biochemistry to create functionalized lipid vesicles, and use (single-molecule) fluorescence microscopy to study their properties.

More about the SigSynCell MSCA Doctoral Training Network

**Qualifications**:
You are an experimental (bio)chemist or molecular biologist with a strong interest in synthetic biology and/or biophysical questions. Experience with either protein purification, handling lipids or fluorescence microscopy would be an advantage. You should like the idea of working in a diverse, collaborative, ambitious and international environment. Excellent verbal and written English skills are essential. You will need to hold an MSc-degree, to ensure eligibility for a Dutch PhD examination.

IMPORTANT: for this position, you must also meet the EU’s “Mobility conditions”:

- MSCA networks require that applicants must not have studied/worked in the host country for more than 12 months in the 3 years immediately prior to the position’s start date.
- This project’s Host Institution is AMOLF, but a part of your doctoral studies will be spent in the laboratory of a consortium member (likely 6 months, the maximum being 12 months).

**Work environment**:
**Working conditions**:

- IMPORTANT: in line with Dutch regulations, this PhD project has a duration of 48 months, i.e. 12 months on top of the 36 months funded by SigSynCell.

**More information?**:
For further information about the position, please contact dr. Kristina Ganzinger:
dr. Kristina Ganzinger
Group leader Physics of Cellular Interactions
Phone: +31 (0)20-754 7100

**Application**:
Please annex your:

- Resume;
- Motivation on why you want to join the group (max. 1 page).

Applications will be evaluated on a rolling basis and as soon as an excellent match is made, the position will be filled.

Online screening may be part of the selection.

**Diversity code**:
AMOLF has won the NNV Diversity Award 2022, which is awarded every two years by the Netherlands Physical Society for demonstrating the most successful implementation of equality, diversity and inclusion (EDI).

**_ Commercial activities in response to this ad are not appreciated._

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