PhD Position in Molecular Mechanisms of Hemostasis

3 weken geleden


Rotterdam, Zuid-Holland, Nederland Erasmus MC Voltijd

Job Description

Von Willebrand Factor (VWF) plays a pivotal role in blood coagulation. VWF is secreted from endothelial cell Weibel-Palade bodies (WPBs), which is crucial for the response to vascular trauma. Dysfunctional WPB biogenesis and exocytosis are characteristic of bleeding disorders such as Von Willebrand disease. In this project, we aim to identify the organelle trafficking routes responsible for WPB formation and secretion.

Research Objectives

This project is a collaboration between the research groups of Dr. Ruben Bierings and Prof. dr. Geert van den Bogaart. Two PhD positions are available, one at each institute.

Methodologies

The Erasmus MC candidate will conduct biochemical assays of WPB cargo secretion and optical assays of WPB exocytosis to determine changes in secretory competence and fusion mode due to altered SNARE sorting. Techniques include high content microscopy, proteomics, total internal reflection fluorescence (TIRF) microscopy, shRNA library screenings, and electron microscopy.

Work Environment

The Department of Hematology at the Erasmus MC has pioneered innovative methods to understand the mechanisms of hematological disease on a molecular and cellular level. Combination of our patient cohort studies with cutting-edge molecular and cellular technologies on patient cells provides the unprecedented opportunity to perform innovative research on the biological mechanisms of disease and to make discoveries that impact the routine practice of patient care.

Qualifications and Skills

To be eligible for this position, you should have a university MSc degree in molecular biology, biochemistry, cell biology, biomedical sciences or any other field that has affinity with basic and/or translational research. Excellent communication and presentation skills are also required. Self-motivated, detail-oriented, and adept at both independent and collaborative work are essential qualities for this position.

Terms of Employment

You will receive a temporary position for 4 years. The gross monthly salary is € 3.017,- in the 1st year and increases to € 3.824,- in the 4th year (scale OIO). Excellent fringe benefits, such as a 13th month that is already paid out in November and a individual travel expense package, are also included.



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