PhD Positions in Molecular Immunology

2 dagen geleden


Groningen, Groningen, Nederland Rijksuniversiteit Groningen Voltijd
Job Description

We are seeking highly motivated PhD candidates to join our research team at the University of Groningen. The successful applicants will be part of a collaborative project between the research groups of Dr. Ruben Bierings and Dr. Geert van den Bogaart, focusing on the identification of organelle trafficking routes responsible for Weibel-Palade body (WPB) formation and secretion.

Research Objectives

The PhD candidates will utilize advanced microscopy methods, including Förster resonance energy transfer-fluorescence lifetime imaging (FRET-FLIM), to map SNARE protein trafficking to WPBs. Techniques will include FRET-FLIM microscopy, mammalian cell culturing, CRISPR, and molecular cloning. The Erasmus MC candidate will conduct biochemical assays of WPB cargo secretion and optical assays of WPB exocytosis to determine changes in secretory competence and fusion mode due to altered SNARE sorting. Techniques will include high content microscopy, proteomics, total internal reflection fluorescence (TIRF) microscopy, shRNA library screenings, and electron microscopy.

Qualifications
  • Master degree in molecular biology, biochemistry, or a related field
  • Good command of English (oral and written)
  • Enthusiastic and a team player
  • Willing to spend research time in Rotterdam/Groningen
  • Experience with mammalian cell biology and advanced microscopy approaches is an advantage
What We Offer

We offer a stimulating scientific environment, access to state-of-the-art devices and techniques, and a beautiful and modern working environment. Employment possibilities will also become available at a spinout company from the laboratory. We offer a salary of € 2,770 gross per month in the first year, up to a maximum of € 3,539 gross per month in the fourth and final year. A full-time position (1.0 FTE) is available, with a holiday allowance of 8% of gross annual income and an 8.3% end-of-the-year allowance. The successful candidate will first be offered a temporary position of one year with the option of renewal for another three years. Prolongation of the contract is contingent on sufficient progress in the first year to indicate that successful completion of the PhD thesis within the next three years is to be expected. A PhD training program is part of the agreement and the successful candidate will be enrolled in the Graduate School of Science and Engineering.



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