PhD Researchers in Transcription Factor Dynamics

4 weken geleden


Leiden, Zuid-Holland, Nederland Universiteit Leiden Voltijd

The Faculty of Science at the Universiteit Leiden, in collaboration with the Leiden Institute of Physics (LION) and the Netherlands Cancer Institute (NKI), is seeking two PhD candidates to investigate transcription factor binding at the single-molecule level.

Precise regulation of transcription is essential for ensuring that the correct genes are expressed in the correct tissue at the correct time. Gene-specific transcription factors (TFs) play a crucial role in activating or repressing transcription of their target genes in specific tissues. Our research aims to uncover how transcription factor binding dynamics and cooperativity are regulated by nucleosomes, self-interactions, and DNA shape, and how these mechanisms affect single-cell transcription dynamics.

We are looking for ambitious and enthusiastic PhD candidates with a strong background in biophysics, molecular biology, biochemistry, or quantitative biology. Previous experience with single-molecule force spectroscopy and/or fluorescence, protein purifications, or in vitro eukaryotic transcription assays is advantageous. The candidates should be creative, independent, and have a problem-solving attitude. Good communication skills and the ability to work in a multi-disciplinary team are essential.

Our research group is part of the Leiden Institute of Physics and the Netherlands Cancer Institute, and we collaborate closely with LUMICKS, manufacturer of C-trap instrumentation. We will setup a novel single-molecule imaging system to measure and perturb the DNA binding kinetics of transcription factors ex vivo and develop a novel assay to map the position of nucleosomes along single chromatin fibers.

We are excited to offer two PhD positions in this exciting and rapidly evolving field. If you are passionate about understanding the mechanisms of transcription factor binding and gene regulation, we encourage you to apply.



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